In-Gel LC-MS/MS

This is the recommended approach for routine protein identification!  It includes in-gel protein digestion, peptide extraction, nanoLC-MS/MS analysis of the digest and a database search using Mascot. Additional bioinformatic analysis will be charged $50/h. The service is a powerful technique for protein identification down to approximately 1 fmol of protein, well below the detection limit of most gel stains.  The thickness of the gel should be 1.0 mm or less. The use of a gel thicker than 1.0 mm will generate non-optimal data. The gel band length (running dimension) must be 6 mm maximum per sample.

Price: $250 per sample

For an economical and handy bulk offering of this service, select “MS-Pass”.

 

In gel MS Pass

A convenient and economical means of accessing In-Gel LC-MS/MS services.   The pass is for 20 samples, is valid for 6 months, and is transferable to other lab members.  The price per sample is $175, compared to $250 per sample if individual gel bands are submitted.  All 20 samples do not have to be submitted at once.  This is our recommended service!

Price: $3500 per pass

In-Solution LC-MS/MS

In-solution digestion is performed instead of in-gel digestion, but samples usually contain contaminants that negatively influence LC-MS/MS analysis.  Our process involves additional clean-up procedures and usually more enzyme to achieve a good result.  We prepurify the digest, then determine the quality of the clean-up using MALDI-TOF.  Then the sample is ready for nanoLC-MS/MS.  Before selecting this service, consult with lab staff for advice on effective sample preparation.

This service can include FASP procedure or on beads digestions.

Price: $300 per sample

In solution MS Pass

A convenient and economical means of accessing In-Solution LC-MS/MS services.   The pass is for 20 samples, is valid for 6 months, and is transferable to other lab members.  The price per sample is $210, compared to $300 per sample if individual sample are submitted.  All 20 samples do not have to be submitted at once.

Price: $4200 per pass

Proteome analysis by 2-Dimension LC-MS/MS

Identify thousands of proteins from your sample. Just provide your cell lysate. The service includes a purification procedure, protein digestion, and two dimensions of peptide separation prior to MS/MS analysis. Proteome comparison between different samples is supported. The service can also be combined with the SILAC technology. Contact us for consultation and a quote.

Price: $900 per sample

This price is typically for 10 fractions per sample.

 

Phosphoproteome analysis by LC-MS/MS

Identify hundreds to thousands of phosphopeptides in your cell lysates. The service includes a purification procedure, protein digestion and peptide enrichment, phosphopeptide extraction, followed by LC-MS/MS for phosphosite determination. The service can also be combined with the SILAC technology. Contact us for consultation and a quote.

Price: $600 per sample

Protein Quantitation - SILAC

Achieve protein quantitation for hundreds to thousands of proteins in your complex samples using SILAC labeling technology!  Use this option for projects that are amenable to metabolic labeling. We provide advice on labeling, assistance in optimizing sample preparation, and of course full nanoLC-MS/MS analysis and data reporting.  Bioinformatics is supported by MaxQuant and Mass Spec Studio. The posted price is per gel band when in gel SILAC LC MS/MS analysis is performed.  We recommend to use 10 gel bands per sample to get a good coverage of a proteome. The final choice about the total  number of gel band used for an experiment is made by the customer. Contact us for consultation and a quote.

Price: $250 per sample

MALDI TOF walk-up

Come and collect your own data using our MALDI TOF/TOF . There is a mandatory training before using this instrument on your own.  The MALDI pass is valid for 6 months and an unlimited number of samples. We provide the MALDI plates and the calibrant. Customers should provide the rest of the consumables.  This mass spectrometer works fine for non complex sample (eg synthetic peptide). We would encourage customer having complex samples to use our in gel or in solution LC MS/MS analysis.

Price: $850 per pass

Self serve service

SAMS could provide detailed protocols to customer interested in processing their own samples to have them “mass spectrometry “ready. For this process, the data would be available within week and the cost reduced. Different protocols are available (In gel, In solution, FASP, on beads digestion). Contact us for consultation and a quote.

Price: $215 per sample