This is the recommended approach for routine protein identification! It includes in-gel protein digestion, peptide extraction, nanoLC-MS/MS analysis of the digest and a database search using Mascot. Additional bioinformatic analysis will be charged $50/h. The service is a powerful technique for protein identification down to approximately 1 fmol of protein, well below the detection limit of most gel stains. The thickness of the gel should be 1.0 mm or less. The use of a gel thicker than 1.0 mm will generate non-optimal data. The gel band length (running dimension) must be 6 mm maximum per sample.
Price: $250 per sample
For an economical and handy bulk offering of this service, select “MS-Pass”.